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Image Search Results


KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Mice hypomorphic for Pitx3 show robust entrainment of circadian behavioral and metabolic rhythms to scheduled feeding

doi: 10.1016/j.celrep.2021.109865

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies tyrosine hydroxylase Aves TYH Goat anti-chicken, AlexaFluor 647 ThermoFisher A-21449 Chemicals, peptides, and recombinant proteins Phosphate buffered saline VWR 97062–730 DAPI Thermofisher D-1306 Paraformaldehyde Sigma P6148 Experimental models: Organisms/strains Pitx3 ak mice The Jackson Laboratory 000942 Oligonucleotides “OR-72F” CTCTCCAGCCTC CCTCAAATACT IDT n/a “OR-75R” TCGGATTTGGCTTCTGATGGTTTT IDT n/a “Pitx3-set3-F” ATTAGAGGTCGTTCAGGATG IDT n/a “Pitx3-set3-R” TAATTGAGGCCTTGGGCTCT IDT n/a Software and algorithms Graphpad Prism 6 Graphpad https://www.graphpad.com Oxymax Columbus Instruments https://www.colinst.com/products/clams-comprehensive-lab-animal-monitoring-system Clocklab Actimetrics, IL Clocklab software Other PicoVac rodent diet Labdiet 5061 Rodent chow Teklad 2018 Metabolic cages and equipment TSE systems TSE phenomaster Activity cages Columbus Instruments CLAMS Whole Body Magnetic Resonance Analyzer System EchoMRI EchoMRI-700 Open in a separate window KEY RESOURCES TABLE Pitx3 ak show free-running circadian rhythms Pitx3 ak mutants entrain behavior to scheduled feeding Pitx3 ak mutants entrain metabolism to scheduled feeding Pitx3 ak mutants accomplish this with a minimal amount of SN dopamine neurons

Techniques: Recombinant, Software, Activity Assay